# Pembrolizumab-scFv Optimiziation Variants Iter0 x PD-1 (YM_0852)

## Overview

YM_852 are Pembrolizumab mutations against its native target; we introduce mutations that include deletions, insertions, double and single mutations to quantify the sensitivity of the local landscape in engaging the native target.

## Experimental details

This dataset includes 29883 unique scFvs and 1 unique native target sequence. A more extensive methods section can be found in our publication [here](https://pmc.ncbi.nlm.nih.gov/articles/PMC12296056/).

## Misc dataset details

We define the following binders:

### A-library (scFvs)

There are several terms you can filter by:

- `WT_<i>`: These are WT replicates.
- `del_<i>`: A deletion from the WT
- `ins_<i>`: An insertion from WT
- `pair_<i>`: A double mutation (2 residues mutated from WT)
- `single_<i>`: A single mutation

To get the mutations of interest relative to the parent, we recommend an alignment to the WT sequence.

### Alpha-library

There is only 1 sequence, which is the native target.