Gene synthesis
Simple Genes
Complex Genes
Gene Clusters
Modules
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Synthesis of gene clusters, gene assemblies and multigenic constructs:

How the Design works:

It uses powerful biocomputation to accomodate all the design requirements, some of which are default and others that are optional or customer-specific.

Ordering the Easy Way    Basically, it is like ordering a la carte in a restaurant:
  • pick your main course and side dishes from our menu,
  • order your selection and
  • let our chefs put together a palate pleaser for you.
  • and enjoy your (multi-)gene assembly

The below graphic gives you an idea of what the design can look like. It can be designed to fully comply with the design parameters of the Toggle plasmid system but it can also be adjusted to any specific cloning / expression system you have, e.g. by considering specific restriction sites and eliminating duplicates computationally from the overall design.

Gene Module Variants

Figure 1.: Gene Cluster design strategy of DNA – assembling with de-assembling option and partial sequence substitution opportunity (ACDCS). X and Y are AscI(BssHII) and MauBI(BssHII) respectively. Combined by ligation BssHII reconstitutes at the position of ligation. This provides the opportunity for to generate multiple BssHII sites at the expression – box junction which further allows you to de-assemble multi-genic constructs (gene clusters) which were generated this way. R1..Rn are sites calculated out of all the coding sequences and also not present in the intergenic regions by design. These are positioned only at desired locations in one of the individual gene constructs for the exchange of specific parts of the intergenic regions (promoters, leaders, RBSs) and individual CDS or variants thereof. 3'-of X and 5'-of Y specific recombination sites are serving for the option of recombination strategies for assembly of pathways.


     

Gene clusters in general

Gene clusters have, on one side, evolved naturally, but can also be designed, e.g. for the purposes of industrial biotechnology. Whether natural or artificial, these assemblies of genes usually code for tightly interacting and functionally coupled enzymes, e.g. those involved in complex, multi-step biochemical processes (catalysis, degradation, etc.). Examples are enzyme cascades involved in amino acid synthesis, the artimisinin biosynthetic pathway, or polyketide synthase complex.

ACDC-SD:

is ATG's assembly cloning - disassembly cloning - substitution design. It is a stringent design that applies a uniform set of constructive principle to genes, gene cassettes and gene clusters. In effect, this means that all modular elements will be fully compatible with other equivalent modules (for the same organism or vector class).